ABSTRACT
High-throughput metabolomics have developed very rapidly in recent years and been widely used in medicinal plants research .At present ,high-throughput metabolomics mainly applied in the following areas ,quality control of medicinal plants by fingerprints ,metabolites difference comparison before and after genetic engineering ,monitoring metabolites change in different environment and gene function study .High-throughput metabolomics have a great future ,but still have some challen-ges ,such as the requirements for more sophisticated equipment and complexity of data integration .With the advancement of science and technology ,high-throughput metabolomics will be an irreplaceable tool for the research of medicinal plants .
ABSTRACT
Baphicacanthus cusia ,widely distributed in the Southwest ,Southern and Eastern regions of China ,is an im-portant medicinal plant of Acanthaceae family .Indigo made from stem and leaf of Baphicacanthus cusia in Fujian has the best quality in China ,and is known as “Jian Indigo naturalis” ,which is the genuine medicinal of Fujian Province .The rhizoma of Baphicacanthus cusia could be used as medicine ,called“Nanbanlangen” ,which together with indigo were included in the“Chi-nese Pharmacopoeia” .Indigo and its original plant both contain indirubin ,which has anti-cancer activity .Indirubin is an active ingredient of Huangdai Pian and Danggui Longhui Wan ,two kinds of traditional Chinese medicine ,which have been successful-ly used in the treatment of malignancies such as chronic myelogenous leukemia .The international advances in the biological characteristics ,genetic diversity ,cultivation technology and molecular biology of Baphicacanthus cusia germplasm resources were summarized .The main problems in Baphicacanthus cusia germplasm resources research are indicated ,which could pro-vide references for the further study and application of Baphicacanthus cusia germplasm resources .
ABSTRACT
The resources and quality of Chinese herbal medicine is the foundation and guarantee for the sustainable development of the whole industry chain of traditional Chinese medicine.The effective component,having clinical curative effect,is the material base of Chinese herbal medicine,and is the core for the quality of medicinal material factors (quality).The accumulation of efficacy material is closely related to the genetic background of the original plant.By clarifying active ingredients biosynthesis,revealing medicinal material quality formation mechanism,regulating the content of effective components,the quality of medicine could be guarantee and ascend.The quality control technology based on active ingredients of Traditional Chinese medicine quality includes integrated analysis of high-throughput sequencing and systems biology data,revealing synthetic genetic mechanisms of active ingredients,excavating molecular markers of the quality,creating excellent germplasm,forming cultivation techniques,establishing classification standard of medicinal materials,and finally realizes the full industrial chain control of Chinese herbal medicine production.Taken all of these together could ensure the quality of medicinal materials.
ABSTRACT
Indole alkaloid family has been the biggest so far among the various alkaloids,which contains pharmaceutical and effective constituents of various plants featuring diverse biological activities.Thanks to the development of metabonomics,to reveal the biosynthetic pathway of active components for the molecular mechanism of indole alkaloids and the regulation research of plant metabolism present a growing importance and significantly direct the researches of improving biological production.This paper reviewed the biosynthetic pathways of some indole alkaloids in accordance with the structure classification of indole alkaloids to lay a foundation for the further studies on the biosynthetic pathways of indole alkaloids and provide a reference for the biosynthetic pathways of other indole alkaloids.